Carangoides bartholomaei (Cuvier, 1833) stomach: a source of aspartic proteases for industrial and biotechnological applications
نویسندگان
چکیده
Abstract The viscera and other residues from fish processing are commonly discarded by the fishing industry. These by-products can be a source of digestive enzymes with industrial biotechnological potential. In this study, we aimed at extraction, characterization, application acidic proteases stomach Carangoides bartholomaei (Cuvier, 1833). A crude extract stomachs was obtained submitted to partial purification process salting-out, which Purified Extract (PE) specific proteolytic activity 54.0 U⋅mg-1. 1.9 fold yield 41% were obtained. PE presents two isoforms maximum 45 °C pH 2.0. stable in range 1.5 7.0 temperature 25 50 °C. kept 35% its presence NaCl 15% (m/v) but totally inhibited pepstatin A. aspartic presented high heavy metals such as Cd2+, Hg2+, Pb2+, Al3+, Cu2+. utilization an enzymatic addictive collagen extraction Nile tilapia scales has doubled yield. results indicate potential these for applications.
منابع مشابه
ارزیابی عادات غذائی گیش ماهی پوزه دراز (Carangoides chrysophrys (cuvier,1833 در آب‌های استان هرمزگان
گیش ماهی پوزه دراز از مهم ترین گونه های خانواده گیش ماهیان (Carangidae) بوده و از ماهیان مهم شیلاتی محسوب می شود. در این پژوهش 440 قطعه گیش پوزه دراز توسط تور ترال صید و از تخلیه‌گاه‌های صیادی که به روش‌های متفاوتی اقدام به صید می‌نمایند از بهار 95 تا زمستان 95 در استان هرمزگان نمونه‌برداری شد. نمونه های ماهیان پس از بررسی اولیه و زیست‌سنجی فریز شده و به آزمایشگاه منتقل شدند. در اب...
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ژورنال
عنوان ژورنال: Brazilian Journal of Biology
سال: 2022
ISSN: ['1519-6984', '1678-4375']
DOI: https://doi.org/10.1590/1519-6984.234413